“If it is a Miracle, any sort of evidence will answer, but if it is a Fact, proof is necessary” -- Mark Twain (American Humorist, Writer and Lecturer. 1835-1910)
Thanks to a post to the Desulfurization Blog by RT@billsimpson19, I found a search engine that finds open access articles … http://www.knowmade.com/free-fulltext-pdf.html
Produced by KnowMade (http://www.knowmade.com/), it eases the search for free full text articles pertaining to your areas of interest.
As easy as it is to acquire full text articles, organizing your e-Library can be a challenge. Corporate librarians and other information professionals use sophisticated software specifically designed for this task. They require such software because they have to keep up with thousands of resources so that they can serve hundreds or thousands of corporate clients.
As an individual, your requirements may be more modest, but no less important. You can purchase a sophisticated program, but you probably can do just as well by maintaining an Excel spreadsheet.
A key concept in organizing your spreadsheet entries is the difference between “find” and “sort.”
In Excel, the “find all” command makes it easy to find a string of characters, such as an author name, no matter where it appears in the sheet. The “sort” function, while important when manipulating financials, is less useful for bibliographic applications. For one thing, it requires far more columns to be useful for sorting.
Take, for example, the following four articles that resulted from a search in the KnowMade database for “desulfurization” …
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Chinese Science Bulletin 2003 Vol. 48 No. 24 2703 2709
Desulfurization metabolite of Rhodococcus erythropolis LSSE8-1 and its related desulfurizational gene fragments
GOU Zhongxuan, LUO Mingfang, LI Xin, XING Jianmin & LIU Huizhou
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China Correspondence should be addressed to Liu Huizhou (e-mail: hzliu@home.ipe.ac.cn)
Keywords: dibenzothiophene, clone, sequence, biodesulfurization, Rhodococcus erythropolis.
DOI: 10.1360/03wb0089
Abstr act Rhodococcus erythropolis LSSE8-1 is a newly isolated biodesulfurizaion strain from the soil of Chishui gas field, Guizhou Province, China. The analysis of its metabolism product shows that the strain is a kind of biocatalyst able to oxidize dibenzothiophene (DBT) to 2-hydroxydiphenyl (HBP), and therefore the sulfur in DBT is selectively removed. By using DBTO (dibenzothiophene 5,5-dioxide) as substrate, both DBT and HBP are found in the culture, which shows that the reaction from DBT to DBTO 2 is reversible in the cell. While using 0.5 mmol/L DBT as control, 0.01 0.4 mmol/L DBTO 2 2 shows poisonous effect to the cell, which will explain why there is no DBTO accumulation in the process of biodesulfurization. After treatment by lysozme, the plasmid DNA of the strain is isolated by alkaline method to be used as the template of PCR reaction. Three dsz gene fragments of 1.3, 1.0 and 1.2 kb respectively were amplified. Each fragment is ligate with PGEM-T vector, and cloned into E. coli.DH5α. The clone DNA is sequenced and the result shows that dsz related genes are highly conservative. The identities of dszAanddszB with respect to IGTS8 are 100%, and the identity of dszC with that of IGTS8 is 99%.
http://www.scichina.com:8080/kxtbe/fileup/PDF/03ky2703.pdf
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Oxidative Desulfurization Using Polyoxometalates
C. Komintarachat and W. Trakarnpruk*
Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn UniVersity, Bangkok 10330, Thailand
Oxidative desulfurization of model compounds (benzothiophene, dibenzothiophene, 4,6-dimethyldibenzothiophene) with hydrogen eroxide/acetic acid using polyoxometalates as catalysts has been studied. The tetrabutylammonium salts of [WO6]192-, [V(VW)O11]404-, [PVWO11]404-, and [PVMo2O10]404-were prepared, and their activities were compared with (VO)2PO. The experimental results show that the highest active catalyst is [V(VW)O11]404-27. The method was also used for the treatment of gas oil. The combination of solvent extraction and alumina adsorption can efficiently separate sulfone products. The resulting oil contained less than 0.055% sulfur, and this corresponds to 90 % removal.
* Author to whom correspondence should be addressed. E-mail: wtrakarnpruk@yahoo.com
http://www.thaiscience.info/Article%20for%20ThaiScience/Article/4/Ts-4%20oxidative%20desulfurization%20using%20polyoxometalates.pdf
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Vol. 45 No. 5 SCIENCE IN CHINA (Series B) October 2002
Isolation and identification of nondestructive desulfurization bacterium
GOU Zhongxuan ( ), LIU Huizhou ( ), LUO Mingfang ( ), LI Shan ( ), XING Jianmin ( ) & CHEN Jiayong ( )
Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China Correspondence should be addressed to Liu Huizhou (email: hzliu@home.ipe.ac.cn)
Abstract A nondestructive desulfurization microorganism has been isolated. The metabolism product analyses show that the strain can be a kind of biocatalyst to oxidize dibenzothiophene (DBT) into 2-hydroxydiphenyl (HBP), therefore the sulfur in DBT is removed selectively. The 16SrRNA information, cell wall analysis, physical, biochemical properties and morphological properties suggest that the isolated strain is Rhodococcus erythropolis. The strain can grow in the basal salts medium (BSM) that DBT concentration is no more than 10 mmol/L, and the optimal DBT concentration for growth is 1 mmol/L, however, the optimal DBT concentration for desulfurization is 0.5 mmol/L. The further research shows that the strain can also desulfur some other organosulfur-containing compounds such as thianaphthene, phenyl sulfide and 4,6-dimethyldibenzothiophene (4,6-DMDBT).
Keywords: dibenzothiophene, isolation, identification, biodesulfurization, Rhodococcus erythropolis.
http://www.scichina.com:8081/sciBe/fileup/PDF/02yb0521.pdf
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Chinese Science Bulletin Vol. 47 No. 13 July 2002
Preparation of microbial desulfurization catalysts
XU Ping, , MA Cuiqing, LI Fuli, TONG Mingyou, ZENG Yiyong, WANG Shuning, H. D. Blankespoor
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;
Fushun Research Institute of Petroleum and Petro-chemicals, China Petroleum & Chemical Corp. (SINOPEC), Fushun 113001, China;
Biology Department, Hope College, MI 49423, USA Correspondence should be addressed to Xu Ping (e-mail: pingxu@sdu.edu.cn)
Abstract A Rhodococcus sp. 1awq, a bacterium isolated from the soil cleaving the C S bond of dibenzothiophene (DBT) via specific pathway, was investigated for cell growth and for its role in desulfurization. Clearly, the end product, 2-hydroxybiphenyl, inhibited the growth of the strain, the synthesis of the desulfurization enzymes, and the activity of the enzymes. The effects of sulfate on the DBT degradation enzymes were examined in the Rhodococcus sp . 1awq growth system with DBT; the sulfate served, concurrently, as the sulfur source. The condition of the resting cells that were used in desulfurization, was also studied. The optimal con-centration of the resting cells and the reaction conditions were determined. It was documented that there is no differ-ence between desulfurization activity for resting cells cultured with sulfate as the sole sulfur source and that with the mixture of DBT and sulfate as the sulfur source.
Keywords: microbe, biodesulfurization, dibenzothiophene (DBT), catalyst.
http://www.scichina.com:8080/kxtbe/fileup/PDF/02ky1077.pdf
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I recommend the easier to maintain Find approach. After all, how many times do you need to sort anyway?
Since it remains true that one picture is worth 1,000 words, I have prepared a small spreadsheet called “FindVersusSort” to illustrate the point. The sheet labeled “Find” shows one possible way of formatting the four articles listed above. The sheet labeled “Sort” shows another.
As you will see, preparing your entries for sorting takes a lot more time than preparing them for finding because of the additional columns you need to provide for each author, corporate source, and keyword. And with all that extra effort, you don’t gain that much. Sure you can sort by author name, but if you have seven author columns, it takes a lot of time to get a meaningful sort.
Send an email to research@jeansteinhardtconsulting.com, and I will send you a copy of the Excel spreadsheet FindVersusSort.xls.
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